Animal Behavior in Armadillidiidae (Pillbug) in Response to Environmental Changes

ABSTRACT: 

In this lab, animal behavior relative to the organism's environment is being observed. In this experiment, Armadillidiidaes (pillbugs) are observed in order to find out their preferences on types of environment. Humidity and acidity/taste are used as two of the factors in this experiment. The results showed that the pillbugs tend to prefer a more moist environment to a dry one. It also revealed that pillbugs highly prefer environments without acidity (basic)

INTRODUCTION:  

Behavior is the way in which one acts or conducts oneself in a set environment. Proximate and ultimate causes also apply to animal behavior. Proximate cause is the immediate trigger for a certain behavior in an animal. Ultimate cause is the evolutionary purpose or reason the animal behaves the way it does. For example in bird song, "why is the bird singing?" would be a proximate question for that certain behavior. An ultimate question for the same behavior would be "what is the evolutionary purpose for a bird to sing?"

http://www.showziji.com/wp-content/uploads/2014/03/wallpapers-bird-singing-animals-nature-landscapes-images-singing-birds-hd-wallpaper.jpg

In ethology, a fixed action pattern is the instinctive behavior that is unchangeable and invariant. These behaviors are produced by a neural network called the innate releasing mechanism in response to an external sensory stimulus called the sign stimulus. This type of behavior is "hard-wired" and instinctive in the animal. 

http://www.realclearscience.com/blog/pato.jpg

Imprinting is a type of psychological behavior best defined as any sort of phase-sensitive learning, which is a type of learning that occurs at a specific age or specific stage in life). It develops rapidly and is completely independent from behavior. Imprinting is described as when an animal or person learns specific behaviors or characteristics of a certain stimulus. A proximate cause for imprinting in young geese is the instinctive and innate response to imprint during the critical period (a time early in animal's life when it forms attachments and develops its own identity) in order to recognize its parent and its own species. The ultimate cause of filial imprinting in young geese is the need to acquire behavioral characteristics from its parent in order to survive and ensure that it will have the best opportunity of minimizing threats of survival (lack of food, predators, etc). 

http://anchaesmicasa.files.wordpress.com/2010/12/imprinting.jpg

Taxis is a specific and directed motion in response to a stimulus. Taxis involves motion in the direction of a stimulus (positive taxis) or away from a stimulus (negative taxis). An example of taxis is when a male moth flies specifically in the direction of a female moth in response to her pheremones so that it can find a potential mate. Kinesis is a random and undirected response to a stimulus. An example of kenesis is when one turns on the lights and cockroaches scatter in any direction. Instead of going towards or away from a stimulus (light), it just causes them to go in whichever random direction. 

http://bio1151b.nicerweb.net/Locked/media/ch51/51_07Kinesis.jpg

A famous example of classical conditioning is Ivan Pavlov's experiment with dogs. Pavlov noticed that dogs began to salivate in response to a sound that has been paired with the presentation or symbol of food. He soon realized that this was a learned response. Classical conditioning involves a neutral stimulus (sound of a bag of dog treats rattling) with an unconditioned stimulus (taste of dog treats). The unconditioned stimulus then naturally triggers an unconditioned response, salivating. It is a type of learned behavior when a conditioned stimulus is paired with an unconditioned stimulus. Operant conditioning, on the other hand, focuses on using reinforcement and punishment to increase or decrease a certain behavior. An example of this is when an owner is teaching a dog how to fetch. If the dog chases after and retrieves the ball, the dog is rewards with a treat as a reward; If the dog fails to retrieve the ball, the owner does not reward it. Over time, the dog learns to associate which of his behaviors receives the desired reward. 

http://static.ddmcdn.com/gif/dog-training-18.jpg

With this in mind, we can have a better understanding in our observation of the pillbugs' behavioral responses to environmental changes such as moisture and acidity. 

HYPOTHESIS:  

If an environment is more moist, then pillbugs will prefer it because they are found mostly under rocks and in moist soil. If the environment is less acidic, then the pillbugs will prefer it because the soil they live in lack acidity and is more basic. In order to test this hypothesis, it is necessary for there to be choice chambers where one does not contain any moisture (control) and one that does. For the acidity/basic test, it is necessary for there to be choice chambers where one does not contain any vinegar (control) and one that does. That way results can be compared to the tested factors. 

 MATERIALS: 

1. 10 Pillbugs
2. Container
3. Cotton
4. Bedding material (for choice chamber)
5. Choice chamber
6. Choice chamber cover
7. Timer

 PROCEDURE:

1. Take a container along with some cotton.
2. Go outside in damp areas (soil, under rocks, etc) to look for 10 pillbugs
3. Place captured pillbugs in the containers and plug the container with cotton so the pillbugs don't escape. 
4. Place thin bedding material in each of the choice chambers.
5. Set  the pillbugs into the choice chambers carefully. 
6. Choose two variables to be tested (for example: moisture and taste). 
7. Observe the first variable by prepping the chambers.
8. Cover the chambers.
9. Check every 30 seconds and record data. 

.

RESULTS: 

CONCLUSION: 

The data collected as a result from this experiment fails to reject my hypothesis. Our data showed that pillbugs preferred moisture (independent variable) to dryness (control). Our data also showed that the pillbugs highly preferred an environment without acidity (vinegar/independent variable) as most of them migrated to the side of the choice chamber without the vinegar (control). The constants in this lab were the temperature, the number of bugs, and the same size of the choice chambers. Potential sources of error may include the pillbugs crawling under the bedding, unable to switch to a desired side, and the death of a pillbug. Both of these errors occurred in our experiment, but our data that we collected was prevailing enough to slighly overlook these errors. 

CITATION: 

 "Proximate and Ultimate Causation" Wikipedia: The Free Encyclopedia. Last modified 14 March, 2014. Accessed 12 April, 2014. http://en.wikipedia.org/wiki/Proximate_and_ultimate_causation

"Fixed Action Pattern." Wikipedia: The Free Encyclopedia. Last modified 2 April, 2014. Accessed 12 April, 2014. http://en.wikipedia.org/wiki/Fixed_action_pattern

"Imprinting." Wikipedia: The Free Encyclopedia. Last modified 21 March, 2014. Accessed 12 April, 2014. 

"My Life as a Turkey: Who's Your Mama? The Science of Imprinting." PBS: Nature. Accessed 12 April, 2014. http://www.pbs.org/wnet/nature/episodes/my-life-as-a-turkey/whos-your-mama-the-science-of-imprinting/7367/

Gleason, Dan. "Imprinting in Birds." Wordpress: Dan Gleason's Blog, Bird Information. Last Modified 2010. Accessed 12 April, 2014. http://dangleason.wordpress.com/avian-biology/172-2/

"Movement: Taxis and Kinesis." Sparknotes: Animal Behavior: Orientation and Navigation. Accessed 12 April, 2014. http://www.sparknotes.com/biology/animalbehavior/orientationandnavigation/section1.html

Cherry, Kendra. "Classical VS Operant Conditioning." About.com: Psychology. Accessed 12 April, 2014. http://psychology.about.com/od/behavioralpsychology/a/classical-vs-operant-conditioning.htm

Immune System Quiz

1.    Provides an immediate nonspecific immune response

a.     The innate, or nonspecific, response immunity is the defense in which humans are born with, protecting them against harmful antigens. It involves barriers including secretory molecules and cellular components. The innate immune system also acts as the first line of defense, present and ready to be action against intruders upon infection. It is not antigen specific and responds equally to a variety of harmful substances without tracking any biological memory of the harmful antigen. Some examples of the nonspecific immune response include the cough reflex, enzyme in tears and skin oils, mucus (traps bacteria and small particles), skin, and stomach acid. It includes the integumentary system (skin/mucus), respiratory system (trachea/nasal passages/lingings) , and digestive system (stomach acids and enzymes.

               

2.    Activates T and B cells in response to an infection

a.     Most of the immune system consists of white blood cells. White blood cells vary in many different types and of those types, the two most prominent are the lymphocytes (T Cells and B Cells). T cells (help activate B cells to divide into plasma cells, call in phagocytes to destroy microbes, and activate killer T cells)  are critical to the immune system and help destroy infected cells and stimulate overall immune response. The T cell possesses a molecule on the surface (T-cell receptor) that interacts with molecules called MHC (major histocompatibility complex). MHC helps the T cell recognize harmful antigens and is also on the surface of most other cells in the body. B cells have the ability to recognize free floating antigens, make antibodies that bind to antigens, and mark the antigen for destruction by cells from the immune system.  B cells can differentiate and produce into antibody. However, they rely on T cells to provide to signal to begin maturation. Once the signal is sent, the B cells produce plasma which in turn secretes large amounts of antibodies.

3.    Responds to a later exposure to the same infectious agent

a.     The adaptive immune system, the specific immune system, acts as second line defense after the innate immune system response and also provides protection to re-exposure to the same infectious agent. Unlike the innate immune system, the adaptive immune system requires some time to react against the invading organism. The adaptive immune system is also antigen specific and reacts only with the organism that induced the response. The adaptive immune system also demonstrates immunological memory, “remembering” (Portion of T and B cells differentiate into memory cells to quickly respond to the same antigen) that it has encountered the invading organism, reacting exponentially faster to the same exposure. Doctors take advantage of this memory to artificially induce defense against an antigen via vaccination.

4.    Distinguishes self from nonself

a.     Every body cell carries distinctive molecules that identifies itself.  The body’s immune system does not usually attack tissues that carry the self-identifying marker. It coexists with other body cells in a state called self-tolerance. When the immune system detects cells or organisms that carry “foreign” molecules, it immediately acts against them. Any tissue or cells from another individual (except from an identical twin) are rejected. This is why patients who taken on transplants must take anti-rejection medicine. The body will even reject proteins unless it is first broken down into its primary non-antigenic building block form. The body detects foreign objects by epitopes which protrude from the surface of the foreign organism. In abnormal situations, however, the immune system may misidentify itself as a foreign substance and carry an immune attack on it. This response is called autoimmune disease. Allergies occure because the immune system is being triggered (cat fur, pollen, etc.) to set off a response.

epinephrine podcast

podcast here

http://answers.yahoo.com/question/index?qid=20110911175440AAnGyrv

http://learn.genetics.utah.edu/content/cells/fight_flight/

http://www.ncbi.nlm.nih.gov/books/NBK21718/

http://e.hormone.tulane.edu/learning/types-of-hormones.html

http://www.buzzle.com/articles/what-is-epinephrine.html

Epinephrine Podcast Transcript

1.      What gland is it produced in?

a.      The inner region, adrenal medulla, of the adrenal glands located on top of both kidneys.

2.      Chemical Pathway – what does this mean/Structure etc.

a.      Cells in the nervous and endocrine system work together in an environment of what our senses perceive as danger, stress, or threat, initiating the fight or flight response.

This elicits instantaneous and simultaneous responses throughout the body

b.      Sensory nerve cells pass the signal to the hypothalamus in the brain. Neurosecretory cells in the hypothalamus transmit a signal to the pituitary gland compelling the cells there to release a chemical messenger into the bloodstream. Simultaneously, the hypothalamus transmits a nerve signal down the spinal cord. The adrenal gland will receive both the chemical messenger and the nerve impulse.

c.      The adrenal glands (located on top of the kidneys) receive the signals initiated by cells in the hypothalamus, activating the release of epinephrine into the bloodstream.

d.      When chemical messengers arrive via the bloodstream, they dock on to receptors and begin cell signaling that results in the production of cortisol. Cortisol is released into the blood stream, resulting in an increase in blood pressure, increase in blood sugar levels, and suppression of the immune system and digestive system.

3.      Water soluble or Fat soluble and Structure – type and contrast it with the opposite type.

a.      Epinephrine is a water-soluble molecule derived from amino acids (the building blocks of protein). These hormones are stored in endocrine cells until they are needed. They act by binding to protein receptors on the outside surface of the cell. The binding alerts a second messenger molecule inside the cell that activates enzymes and other cellular proteins or influences gene expression.

b.      Most water-soluble hormones can travel freely in the blood because they "like" water. They are repelled by lipid and fatty structures such as the membranes that surround a cell and its nucleus. Because of this repulsion, these hormones generally bind to receptor sites on the outside of the cell and signal from there.

c.      Fat-soluble hormones, like the sex hormone steroids estrogens and androgens, are fat soluble and water repellent. They "like" lipid or fatty structures (such as those surrounding cells and are repelled by watery areas). Steroids generally travel to their target cells attached to a special carrier protein that is water soluble. (such as, sex steroid hormone binding globulin and serum albumin). The hormones detach before passing into the cell where they bind to receptors.

4.      Type of cellular receptor

a.      Epinephrine, which binds to two types of GPCRs, is important in mediating the body’s response to stress, when all tissues have an increased need for glucose and fatty acids. These principal metabolic fuels can poured into the blood in a matter of seconds by the breakdown of glycogen in the liver and of triacylglycerol in adipose storage cells.

b.      “In mammals, the liberation of glucose and fatty acids can be triggered by binding of epinephrine (or norepinephrine) to β-adrenergic receptors on the surface of hepatic (liver) and adipose cells. Epinephrine bound to similar β-adrenergic receptors on heart muscle cells increases the contraction rate, which increases the blood supply to the tissues. Epinephrine bound to β-adrenergic receptors on smooth muscle cells of the intestine causes them to relax. Another type of epinephrine receptor, the α2-adrenergic receptor, is found on smooth muscle cells lining the blood vessels in the intestinal tract, skin, and kidneys. Epinephrine bound to α2 receptors causes the arteries to constrict, cutting off circulation to these peripheral organs. These diverse effects of epinephrine are directed to a common end: supplying energy for the rapid movement of major locomotor muscles in response to bodily stress. As discussed in more detail later, β- and α-adrenergic receptors are coupled to different G proteins. Both β1- and β2-adrenergic receptors are coupled to G proteins (Gs), which activate adenylyl cyclase. In contrast, α1 and α2 receptors are coupled to two other G proteins, Gq and Gi, respectively. Gi inhibits adenylyl cyclase, and Gq stimulates phospholipase C to generate IP3 and DAG as second messengers.”

5.      What type of feedback loop is it?

a.      Positive.

6.      Action of hormone or its function – specific

a.      It regulates heart rate, blood vessel, air passage diameters, and metabolic shifts. It also controls our instinctive fight or fight response.

b.      After epinephrine is released, the supply of oxygen and glucose to muscles and the brain is increased. There is a rise in stroke volume (the volume or quantity of blood pumped by a ventricle due to a single heartbeat) and the heart rate increases. The hormone also dilates the pupils and constrict the arterioles present in the skin. The secretion of adrenaline also brings up the sugar level in the blood as well as suppressing the function of the immune system and digestive system.

7.      What is this regulated by?

a.      Epinephrine is regulated by the central nervous system. The sympathetic nervous system and the Adrenocorticotropic hormone both play important roles in the synthesis of epinephrine.

Take Home Quiz

Case: A twenty-year old man was found supine on Thompson Creak Trail with a bullet wound. The entrance of the wound was on the left lateral side 1cm above the third rib. The exit wound was 5cm above the belly button in the umbilical region. While tracing the bullet path you notice at the entrance the bullet travels in the frontal plane with a 45-degree downward angle. On inspection of the right side of the rib cage by x-ray you observe the 8th rib fractured. Fragments of the bullets are then traced to the final exit wound.

3 scenarios

-The man was shot in the heart and died of organ failure. The heart is unable to function and pump blood into the organs necessary for human life.

-the fragmented bones (from when the man fell after being shot) of from the fractured rib (8th rib) damaged the liver and nearby organs (such as the lung and the stomach), causing massive internal bleeding.

-The bullet may have puncture the lung and caused blood to spill into the victims lungs, causing him the inability to breath.

Ted Talk: Anthony Ayala

Ted Talk: Anthony Ayala

• 1950s - 1st ever organ transplant (kidney)
• number of people needing transplants doubled as time progressed but number of organs available remained the same. This is due to longer human lifespan. 
• Every 30 secs, a patient dies from diseases that could be treated with tissue transplant. 
• Bone regenerates every 10 years. Skin regenerates every 2 weeks.
• Body's first reaction to injury is to seal away injury (organs, skin, etc). Scar tissue.
• Materials can be used as bridge to regenerate tissue. 
• Max amount body can regenerate is 1cm.
• Injured patient -> take small part of tissue -> tease tissue and cells out (separating) -> grow in large quantity. Use scaffold to bring cells into body to regenerate new tissue. Once tissue regenerates, scaffold disintegrates. 
• Engineered organs (muscle, blood vessel) using scaffold must be exercised for use in human body. 
• Can use printer to print organs. Cells instead of ink.
• Can use decellularized organ. Take donated organ, strip the cells, take cells and put it on the "skeleton" of cell. Use to create functional organ.
• 90% people waiting for organs are waiting for kidneys.
• Your own cells will not be rejected from the body. 
• Stem cells

Your Inner Healers Article

iPSC (induced pluripotency stem cells) - stem cells produced from regular cells of an organism

• Shinya Yamanaka of University of Kyoto (2006) revealed their formula for creating the iPSC. 
• Scientists are working to develop this in order to gain more understanding on diseases that have evaded cures (diabetes 1, alzheimer's, parkinson's). 
• The ability of being able to change a cell's identity is revolutionizing the way scientists look at human development. 
• Although sophistication of technology has not yet reached the ability for humans to theoretically become immortal, it is a possibility that may ignite more ethical issues that embryonic stem cells.

Primordial Power

• differentiation: when cells become less versatile and more specialized as time goes on. (mammal)
• embryonic stem cells have the ability to become 220 different cell types. 
• multipotent: cells which can only develop into specific families of cells types (muscle/bone)
• adult "stem cells" just replace mature cells within a tissue. 
• cells do not revert back to more primitive cell (in mammals) with the exception of cancer. 
• cellular reprogramming: tricks mature cell to act as an embryonic cell. (cell nuclear transfer "cloning")
• cloning: injecting adult cell into egg cell who's genetic material has been removed. 
• attempts to create embryonic cells from human clone cells have failed. 
• nuclear transfer looked into as possible way for replacing damaged cells/tissue. 
• Yamanaka introduced genes normally active only in embryos into adult cells. 
• introduced gene with retroviruses -> cell reprogrammed into pluripotent cell 
• can avoid ethical and legal contraints

Identity Crisis

• iPSC may look like embryonic stem cells and display molecular markers associated with pluripotent cells. 
• pluripotency of a cell: ability of stem cells to produce a wide variety of body cell types in a petri dish, ability to produce teratoma (tumor containing cells from embryonic tissue lineages) when injected under skin of mouse, and capacity (when injected in mouse embryo) to contribute to development of tissue lineages. embryonic stem cells usually pass test, iPSC do not. 
• viruses developing programming genes usually are not properly shut off. Important genes are not properly turned on. 
•